Mechanism of action of stabilized stannous fluoride dentifrice

VSCs are the bacterial byproducts of metabolic activity, especially in anaerobic Gram-negative bacteria that proliferate on the tongue. Stannous fluoride exerts its anti-bacterial effect, primarily through metabolic inhibition. Ultimately, this leads to a reduction in the production of VSCs. Stannous ion can also bind directly to the sulfur sites in the sulfur-containing metabolic substrates (e.g. the sulfur-containing amino acids methionine and cysteine) creating competitive antagonism for their metabolism. The net effect of either mechanism of action is to reduce the level of foul-smelling VSCs (Figure 17)..

The following study summaries represent a sample of research demonstrating the benefits of stabilized stannous fluoride dentifrice for reduction of breath malodor.

EFFECTS OF 0.454% SNF2 DENTIFRICE ON DAYTIME AND OVERNIGHT MALODORL

Reference: Farrell S, Gerlach RW, Barker ML, et al. J Dent Res. 2008;87(spec issue B): abstract 3161.

OBJECTIVE

A clinical study was conducted to evaluate daytime and overnight oral malodor reduction benefit of a 0.454% stabilized stannous fluoride therapeutic dentifrice with short-term and long-term use.

MATERIALS AND METHODS

• The study was a randomized, double-blinded, 2-treatment, 3-period crossover clinical trial.
• After completing an acclimation period, 45 subjects with existing oral malodor were randomly assigned to a crossover treatment sequence consisting of Crest^® PRO-HEALTH^™ dentifrice (0.454% stabilized stannous fluoride dentifrice) and Crest^® Cavity Protection dentifrice (control).
• For each treatment period, subjects brushed with the assigned product twice a day for 7 days. Oral malodor was assessed on a 9-point hedonic scale at baseline, day 2–overnight, day 2–daytime (4 hours post morning brushing), day 8–overnight, day 8–daytime (4 hours post morning brushing). Treatment periods were separated by washout periods during which subjects brushed with the control dentifrice.

RESULTS

• Subjects had a mean age of 39 years, 58% of the subjects were female and the mean baseline hedonic score was 7.4.
• Relative to the control, use of the stabilized stannous fluoride dentifrice resulted in significant (P<0.002) improvement of the overnight and daytime malodor both short-term at day 2 and long-term at day 8.
• The mean overnight hedonic scores were 3.2 and 5.1 at day 8 after 1 week of brushing for the stabilized stannous fluoride and the control dentifrices, respectively. The mean daytime hedonic scores were 2.4 and 4.1 at day 8 for the stabilized stannous fluoride and the control dentifrices, respectively.

ORAL MALODOR REDUCTION WITH 3-WEEK USE OF 0.454% SNF2 DENTIFRICE

Reference: Nachnani S, La S, Lee S, et al. J Dent Res. 2008;87(spec issue B):abstract 2864.

OBJECTIVE

This clinical study evaluated the effects of the 3-week use of a 0.454% stabilized stannous fluoride therapeutic dentifrice on oral malodor.

MATERIALS AND METHODS

• The study was a randomized, double-blinded, 2-treatment, parallel design clinical trial.
• After completing an acclimation period, 71 subjects with existing oral malodor were randomized to 1 of the 2 treatments: 0.454% stabilized stannous fluoride dentifrice (Crest^® PRO-HEALTH^™) or Crest^® Cavity Protection dentifrice (control). Subjects brushed with the assigned product twice a day for 3 weeks.
• Oral malodor was assessed on a 9-point hedonic scale at baseline, week 1, and week 3.

RESULTS

• The mean age of study participants was 37.8 years, and 59% were female. The baseline mean hedonic score was 8.19.
• At week 1, the mean hedonic scores (SE) were 3.40 (0.18) and 6.62 (0.18) for the stabilized stannous fluoride dentifrice and the control dentifrice, respectively.
• At week 3, the mean hedonic scores (SE) were 1.55 (0.18) and 5.28 (0.18) for the stabilized stannous fluoride dentifrice and the control dentifrice, respectively.
• Relative to the control, the use of the stabilized stannous fluoride dentifrice resulted in significantly (P<0.0001) greater reduction in oral malodor at both visits. Both treatments were well tolerated.

CLINICAL SIGNIFICANCE: ORAL MALADOR